A recombinant DNA was constructed by inserting the DNA of in

A recombinant DNA was constructed by inserting the DNA of interest into the Amp^R gene of a pBR322 vector. The vector also contains a Tet^R gene that is not interrupted. After introducing the recombinant DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected?

Solution

The following results would be expected:

B)

growth on ampicillin but not tetracycline containing media

As due to insertional inactivation growth will be seen in case of ampicillin but not in case of tetracycline containing media as the Tet^R gene that is not interrupted.