researchers studying tissues of diseased marine mammals have

researchers studying tissues of diseased marine mammals have identified what appear to be a viral particles by electron microscopy. describe three methods you might use to attemp to culture this virus for further study

Solution

The key for better understanding the viral particles and to grow and use them in lab is to look for the host-pathogen interaction. Also we can have robust human cell culture in 3D which could be helpful replicating and recapitulating tissue architecture thus providing the microenvironment. There are many animal models to use the viral particles and tests its virulence, but here we will see few modern techniques to have the mammalian viral particles isolated and cultured. Here in this section while giving three methods we will mainly talk about the Bioreactors and the use of in vitro cell culture. To overcome the monolayer cell culture few research groups have found out few modern techniques those are mentioned below.

i) Culturing viral parties with human tissue aggregates in the rotating wall vessel (RWV) bioreactor. Here a monolayer of cells are used on them there are microcarrier beads and appropriate media with constant rotation. Simulated microgravity environment is to overcome sedimentation and attachment onto the walls finally forming the microparticles. A wide variety of cell lines like Lung, neuronal, cervical also vaginal tissues could be used. 3D models of Human Neuronal cells are used to study Persistence Viral infection.

ii) We can have molecular techniques like Real-Time (RT) PCR, for example in case of Influenza virus, this detection provides a lot faster, accurate end-point analysis, also have better sensitivity than culture. Also this is unconventional in way that it is difficult to make the master mix also it is costly. On the other hand a research group at Lawrance Livermore National Laboratory have detected virus using probes against genomic DNA sequence within 24 hours.

iii) Immunofluorescence assays are widely used for rapid detection from the viral particle samples in any clinical specimen. Overall this techniques gives a sensitive and specific viral identification. This techniques helps identifying viral antigens.