I need a phosphate buffer with a pH of 75 and I have NaH2PO4
I need a phosphate buffer with a pH of 7.5 and I have NaH2PO4 (acidic salt) and Na2HPO4 (salt with conjugate base) available. What concentration of each do I need? Hint: Use the Henderson-Hasselbalch equation below. Set acid concentration to 1 The pKa of H2PO4- is 7.21.
Solution
pH = pka +log (acid/salt )
7.5 =7.21 +log (acid/salt )
(acid/salt ) =e^0.29=1.336
acid conc = 1.336 salt conc

