I also add the DNA seq For 2nd basepair position of all such

For 2nd base-pair position, of all such (2 base) mutations are synonymous For 3rd base-pair position, of all such (3rd base) mutations are synonymous Part C (5 points): What would be the effect on the amino acid sequence ofinserting a single base, G, between sites 10 and 11 in the DNA sequence above? Name the process involved and show your work. Part D (5 points: What would be the effect on the amino acid sequence of deleting nucleotide 16? Name the process involved and show your work

Solution

PART C.

Given DNA sequence is CCCACGACAGAACAGTATTTAAGGAGCTGCGAAGGT which codes for

P T T E Q Y L R S C E G

When the G is added between the 10 and 11 nucleotide new sequence will be CCCACGACAGgAACAGTATTTAAGGAGCTGCGAAGGT which will code for

P T T G T V F K E L R R

As we can clearly see from the translated product that upon addition of G entire amino acid sequence is changed.

The process involved is called Frameshift mutation which is due to insertion of one nucleotide in the sequence

Part D.

Given DNA sequence is CCCACGACAGAACAGTATTTAAGGAGCTGCGAAGGT which codes for

P T T E Q Y L R S C E G

When the 16^th nucleotide is deleted new sequence will be CCCACGACAGAACAGATTTAAGGAGCTGCGAAGGT which will code for

P T T E Q I Stop G A A K

Deletion of nucleotide shift the entire frame and due to that stop codon id formed which will end the translation prematurely and as a result of it, will get peptide of fewer amino acids