Why do you think it is important to do spotinoculations for

Why do you think it is important to do spot-inoculations for the amylase test and the caseinase test? What happens if you drop H_2O_2 into a cut on your arm? What does this tell you about the type of enzymes your cells might produce?

Solution

Answer:

1. Spot inoculations are a means of conveniently inoculating in such a manner that all growth radiates outward from approximately a single point, i.e., such that a single colony is formed.

A spot inoculation is done to ensure that a lot number of bacteria grow at a single point and produce concentrated amounts of the enzymes, amylase or casienase to judge activity.

2. Hydrogen peroxide bubbles when it comes into contact with an enzyme called catalase. Most cells in the body contain catalase, so when the tissue is damaged, the enzyme is released and becomes available to react with the peroxide.

Catalase allows hydrogen peroxide (H₂O₂) to be broken down into water (H₂O) and oxygen (O₂).