ELISA question Why did you need to wash the wells after ever

Solution

Correct washing is essential for succussful ELISA. In ELISA each and every step completion, there is need of Washing using wash buffer. Wash buffer generically diluted in distilled water. Then used it for washing. This washing eliminates unbounded components like unbound primary, secondary antibodies and prevents false result.

Washing helpful for the seperation of Bound and non bound reagents in Wells of ELISA plate