Which protein IgG or BSA binds Coomassie dye better and whyS

Which protein IgG or BSA binds Coomassie dye better and why?

Solution

BSA is a good binder.

Further

Each of the commonly used total protein assay methods exhibits some degree of varying response toward different proteins. These differences relate to amino acid sequence, isoelectric point, structure and the presence of certain side chains or prosthetic groups that can dramatically alter the protein’s color response. Most protein assay methods utilize BSA or immunoglobulin (IgG) as the standard against which the concentration of protein in the sample is determined.BSA provide a consistent standard for protein estimations. Nevertheless, individual proteins, including BSA and IgG, differ slightly in their color responses in the Coomassie Assay . For greatest accuracy, the standard curve should be prepared from a pure sample of the target protein to be measured.