Homework SourseAnalyzing a Metabolic Pathway How The Beadle and Tatum Exper
Analyzing a Metabolic Pathway
How The Beadle and Tatum Experiment Worked
An experiment was performed in order to elucidate the biosynthetic pathway of the formation of histidine by eukaryotic organisms. Scientists found a species of fungi which demonstrated several mutations within the genes which code for the enzymes responsible for the conversion steps for this reaction. Scientists knew that the intermediate products in the reactions were the following:
CAT MAT RAT FAT SAT BAT
However, they did not know the order in which they were formed in the reaction. They also knew that one enzyme was responsible for each of the steps of the conversion. They grew cultures of each of the mutants on slants containing each of seven media (Fig.B). The results are recorded in the Data Table below. When there was growth of the fungus on the medium, the scientists recorded a + in the box.
DATA TABLE:
Minimal
Media (MM)
MM/
CAT
MM/
BAT
MM/
RAT
MM/
FAT
MM/
SAT
MM/
MAT
Mutant 1
+
Mutant 2
+
+
+
+
+
Mutant 3
+
+
Mutant 4
+
+
+
+
Mutant 5
+
+
+
Mutant 6
+
+
+
+
+
+
Wildtype
+
+
+
+
+
+
+
Complete the metabolic pathway below by analyzing the results in the table. In the top blanks, record the # of the mutant with the non-functioning enzyme for that conversion (EX: __ #5 )
In the bottom blanks you will place the names of the intermediates in the order they occur in the reaction.
Precursor à __________ à__________ à __________ à _________ à __________ à _________
| Minimal | MM/ | MM/ | MM/ | MM/ | MM/ | MM/ | |
| Mutant 1 | + | ||||||
| Mutant 2 | + | + | + | + | + | ||
| Mutant 3 | + | + | |||||
| Mutant 4 | + | + | + | + | |||
| Mutant 5 | + | + | + | ||||
| Mutant 6 | + | + | + | + | + | + | |
| Wildtype | + | + | + | + | + | + | + |
Solution
Answer :
RDER OF PATHWAY :
WILD type --------- > Mutant 6 ---------> Mutant 2 -----------> Mutant 4 --------> Mutant 5 -------> Mutant 3 -------> Mutant 1
