Why do we analyze the digested DNA samples pUC19 and GFPuv P

Why do we analyze the digested DNA samples (pUC19 and GFPuv PCR product) before performing ligation?

Why is the DNA fragment amplified with Phusion DNA polymerase can’t be directly cloned into PGEM-t plasmid?

Solution

que-1: Why do we analyze the digested DNA samples (pUC19 and GFPuv PCR product) before performing ligation?

Answer:

It is essential to analyze \"restriction endonuclease- digested DNA samples of plasmids -pUC19 and GFPuv\" to observe transformation of genes into the vector or to observe recombination frequency of genetic insert prior to DNA ligation. This analysis can be done using PCR followed by 1% Agarose Gel Electrophoresis finally it is possible to differentiation recombinant or non-recombinant DNA constructs