In an experiment similar to the Lederberg and Tatum experime

In an experiment similar to the Lederberg and Tatum experiment, researchers grew two strains of E. coli in complete medium. E coli strain AX has the genotype Amp^sbio + trp + leu-. E coli strain AY has the genotype Amp^R bio- trp - eut. The researchers then plated the bacteria on different media types. Next, the researchers mixed equal amounts of the AX and AY E. coli strains. On which type(s) of media would growth provide evidence that genetic transfer had occurred between the two strains of bacteria? Select all that apply. bio- leu- bio- trp- trp- leu- leu- trp- bio- none of the above

Solution

ANSWER OF FOLLOWING QUESTION -

option 2 is correct

Bacterial medium should be deficient bio^- typ^{- to} prove that genetic transformation from strain AX to AY.

Because the genotype of AX amp^s bio₊ typ+ lec- and the genotype of AY is amp^r bio- trp-Leu+.

Now if gene transfer from Genotype AX to genotype AY become amp^r bio+ typ+ leu+ then AY genotype does not require Biotin and tryptophan in medium.