Homework SourseCalculate the gram amount of imidazole and volume of 10X PBS
Calculate the gram amount of imidazole and volume of 10X PBS and water to use to make 10mL of a solution that is 1X PBS and 500mM imidazole
Calculate the gram amount of imidazole and volume of 10X PBS and water to use to make 10mL of a solution that is 1X PBS and 25mM imidazole.
Solution
Ans. Part 1:
# Required moles of imidazole = Molarity x Volume of solution in liters
= 0.500 M x 0.010 L
= 0.005 mol
Required mass of imidazole = Required moles x Molar mass
= 0.005 mol x (68.077 g/ mol)
= 0.1159 g
# Using C1V1 (10X PBS) = C2V2 (1 X PBS)
Or, (10X x V1) = (1X x 10 mL)
Or, V1 = (1X x 10 mL) / 10X = 1.0 mL
Therefore, required volume of 10X PBS solution = 1.0 mL
# Required volume of water = Total volume – Volume of 10X PBS taken
= 10.0 mL – 1.0 mL
= 9.0 mL
Note: It’s assume that addition of imidazole does not affect the volume of solution.
Part 2:
# Required moles of imidazole = Molarity x Volume of solution in liters
= 0.025 M x 0.010 L
= 0.00025 mol
Required mass of imidazole = Required moles x Molar mass
= 0.00025 mol x (68.077 g/ mol)
= 0.0170 g
# Using C1V1 (10X PBS) = C2V2 (1 X PBS)
Or, (10X x V1) = (1X x 10 mL)
Or, V1 = (1X x 10 mL) / 10X = 1.0 mL
Therefore, required volume of 10X PBS solution = 1.0 mL
# Required volume of water = Total volume – Volume of 10X PBS taken
= 10.0 mL – 1.0 mL
= 9.0 mL
Note: It’s assume that addition of imidazole does not affect the volume of solution.
