2Omethylation is a common regulatory modification in certain

2’-O-methylation is a common regulatory modification in certain nucleic acid structures. Which of the processes shown in the figure would be affected by 2’- O-methylation?

Please explain, thank you for your help!

O-P-OH Base O-P-OH O-P-OH Base Base O-P-OH Base OH OH OH OH PCO O FP-OH HO-P-O O P-OH PP O-P-OH O-P-OH O-P-OH Base Base Base OH H OH H PP O P-OH

Solution

(ii) process would be affected due to 2\'O methylation beause in the given three pprocesses that is tje only one in which 2\' OH is being used in the bond formation while in other two there is no use of 2\' OH.In (ii0 process 2\' OH will be modified by methylation hence it will not be recognised by the enzyme responsible for the reaction and reaction will be affected.