The student does an experiment to identify B cells and helpe

The student does an experiment to identify B cells and helper T (TH1) cells in the lymph nodes of MCMV-infected mice. She isolates lymph nodes (LN), selects the lymphoid cells with low FSC/SSC. and then attempts to identify B cells and TH1 cells by a series of flow (FACS) experiments. Describe the experimental scheme below that will identify B and T cells (left plot), and TH1 cells (right plot). Label/circle all the expected cell populations on the FACS plots:

Solution

Forward scatter is with respect to size whereas side scatter is due to granularity of cells.

Flow cytometry technique is used by passing light which streamlines the cells by hydraulic phenomenon and thus helps in cell identification,cell sorting and all..

So population-1 belongs to T lymphocytes

Population -2belongs to B lymphocytes In the left plot

In the right plot...-

Population 1- T helper cells.

Population 2- Tregulatory cells