Explain why the enzymatic activity of creatine kinase and la

Explain why the enzymatic activity of creatine kinase and lactate dehydrogenase is measured indirectly using NADH and. NADPH

Solution

The enzymatic activity of Creatine kinase can be determined spectrophotometrically; by measuring NADPH formed by the enzyme hexokinase and the glucose- 6-phosphate dehydrogenase coupled enzymatic system; while the enzymatic activity of lactate dehydrogenase can be measured directly by NADH consumption. This is based on the principle that; reduced NAD + i.e. (NADH) absorbs UV strongly at 340 nm while the oxidized form doesn’t.