Using a spectrophotometer you quantitated the enzyme reactio

Using a spectrophotometer, you quantitated the enzyme reaction by measuring the absorbency from the oxidized OPD at 410nm. The more colored OPD produced in a given time period, the faster the enzymatic reaction. The following are the contents of experimental test tubes (Table 1) and the absorbance readings. A. Write a hypothesis for this experiment. B. What is the substrate of the enzyme peroxidase in this experiment? C. Which test tube contains the experiment designed as \"no enzyme control\"

Solution

(A) The null hypothesis can be stated as: \'Rate of oxidation of hydrogen peroxide does not increase with enzyme (peroxidase) activity\'

In this experiment, you would falsify the null hypothesis by proving that since the absorption values of the dye increased with increasing enzyme concentration, the oxidation rate also increases.

(B) The substrate of the enzyme peroxidase in this experiment is Hydrogen peroxide (H₂O₂).

(C) The test tube \'Blank\' lacks any enzyme. Therefore, it is the test tube designed as \'no enzyme control\'.