Look at the DNA sequence below and observe the effect of Eco

Look at the DNA sequence below and observe the effect of EcoRI digestion: What nucleotides does EcoRI cut between in the palindromic sequence GAATTC? How many base pairs are there to the left of the \"cut\"? How many base pairs are there to the right of the \"cut\"? Counting the number of base pairs, is the right fragment the same size as the left fragment? How could you describe the size of each fragment in terms of the number of base I pairs in the fragment?

Solution

1. EcoR1 cuts between the Guanine (G) and Adenine (A) nucleotides in the sequence.

2. 4 basepairs are there to the left of the cut. (G-C, T-A, A-T, C-G)

3. 10 basepairs are there to the right of the cut. ( C-A, A-T, T-A, T-A, C-G, A-T, C-G, G-C, C-G, A-T)

4. No, the number of base pairs in the right of the fragment is not same as the size of left fragment.

5. If the number of basepairs are more then the size of the fragment will be more. It can described in terms of Kilobases (Kb) or basepairs.