To be completed prior to lab on a separate sheet of paper Pl

To be completed prior to lab on a separate sheet of paper. Please read through the lab manual and answer the following questions. Please indicate on which page of the lab manual the answer was found. What is the purpose of the InstaGene matrix added to your DNA? How many times will you add saline, spin, and discard the supernatant?

Solution

1. The insta gene matrix is added to DNA in PCR method to enhance the efficiency of DNA amplification by PCR. This matrix binds to PCR inhibitors rather than DNA; this prevents the loss of DNA due to irreversible binding. the matrix absorbs the cell lysis products and DNA found in the supernatant is ready to use in PCR.

2.Saline is added once, spinning is done for several times and the supernatant must be discarded asmuch as possible