Plants use specialized membraneenclosed organelles called le

Plants use specialized membrane-enclosed organelles called leucoplasts to store starch, lipids, and proteins. You predict that these organelles utilize a similar method for importing proteins from the cytosol as the endoplasmic reticulum. Describe how you would determine whether the following peptide sequence serves as a leucoplast localization sequence^N\'-MAILFVFWYIAAYYLVWILL-^c\'

Solution

The gene with this peptide sequence will be designed and amplified by PCR and cloned into an vector. This will be GFP labelled which will help in identification of its traffeking. Leucoplast suspension will be made from the plant and the protein will be added to the culture and the localisation will be imaged.