Homework SourseBelow is a table that lists a sequence of nucleotides and th
Below is a table that lists a sequence of nucleotides and their probability of occurring in a particular position in the bacterium, E. coli. This sequence is significant for directing a key life process.
T
A
T
A
A
T
82%
89%
52%
59%
49%
89%
What is this sequence called?
What is indicated by the percentage values?
What key life process is this sequence important in?
What is its role in the process you stated above?
What protein is mainly responsible for recognition of this sequence?
| T | A | T | A | A | T |
| 82% | 89% | 52% | 59% | 49% | 89% |
Solution
The TATAAT sequence in E.coli is called PRIBNOW BOX. it is a six nucleotide long sequence located 10 basepair upstream from the transcription initiation site.
the percentage value indicate the probablity of occurence of each nucleotide in E. coli.
the key life process transcription is dependent on this sequence, transcription is the synthesis of mRNA from DNA.
IT is a consensus sequence it shows the most frequently occuring base at each position in a large no of promoters analysed. it is important in transcription initiation.
RNA POLYMERASE the main enzyme of transcription is responsible for recognition of this sequence.
