Given the information below which of the following statement

Given the information below, which of the following statements is CORRECT?

9.6

A) Given a mixture of Protein A and Protein B, ion exchange chromatography can be used to separate the two proteins

B) Given a mixture of Protein A and Protein C, gel filtration chromatography can be used to separate the two proteins

C) Given a mixture of Protein B and Protein C, gel filtration chromatography or ion exchange chromatography could be used to separate the two proteins

D) Given a mixture of Protein B and Protein C, gel filtration chromatography can be used to separate the two proteins but ion exchange chromatography could NOT be used to separate the two proteins

Solution

Both ion exchange chromatography and gel permeation chromatography can be used to separate proteins. In ion exchange chromatography, proteins are separated according to the affinity of charges on proteins to the solid supports. In gel permeation chromatography proteins are separated according to their size. Smaller proteins diffuse unto the gel beads and move slowly while larger protein molecules elute first.