GSSGNADPHH2GSHNADP link with table 141 httpswwwcheggcomhomew

GSSG+NADPH+H+?2GSH+NADP+

link with table 14.1: https://www.chegg.com/homework-help/questions-and-answers/calculate-g-reaction-using-e-0-values-table-141-textbook-g-value-atp-hydrolysis-322-kj-mol-q19901236

Part A Calculate AG for the glutathione reductase reaction in the direction shown, using Eo\' values from Table 14.1 in the textbook. Express your answer with the appropriate units. kJ AG 17.4 Submit My Answers Give Up Correct Part B Suppose that a cell contained an isoform of glutathione reductase that used NADH instead of NADPH as the reductive coenzyme. Would you expect AG\" for this enzyme to be higher, lower, or the same as the corresponding value for the real glutathione reductase? AG\"would be higher. AGO would be lower AG would be the same Submit My Answers Give Up Correct

Solution

Answer:

Part-A:

                 NADPH undergoes reversible reaction and gives NADP⁺, H⁺ AND 2e^-. Here,   Eo\' (V) = -0.32.

    Oxidized form of Glutathione is reduced by accepting two hydrogen atoms and two electrons. Here, Eo\' (V) = -023.

By using the equation G⁰\' = -nFE⁰\' to calculate the G⁰\'

Here, n = 2

         F = Farady constant (96485.33)

    G⁰\' = -2(96485) [-0.23- (-0.32)]

              = -192970[0.09]

              = 17367.3J/mol

             = 17.37 KJ/mol

The G⁰\' value for the glutathione reductase reaction is -17.37 KJ/mol.

Part-B:

     The G⁰\' value for the given reaction is same as above i.e., -17.37 KJ/mol. This is because, E⁰\' value is same for NAD⁺/ NADH and NADP⁺/NADPH.

Part-D:

The G value would be probably positive when it is works with nicotinamide adenine dinucleotide (NADH), because if the concentration ratio of NAD⁺/ NADH is high, then oxidation of glutathione occurs.