A phageinfected bacterial culture was subjected to a series

A phage-infected bacterial culture was subjected to a series of dilutions, and a plaque assay was performed in each case, with the results shown in the following table. In the plaque assay using the 10^6 dilution. What is the initial concentration of phage assuming that 0.1 ml was used in the assay? Enter your answer to two significant digits.

Solution

Answer:

Dilution factor: 10^6

Volume plated: 0.1 mL

Number of plaques: 11

plaques/ml = (no. of plaques x dilution factor) /volume of culture plate

=(11 x 10^6) / 0.1 mL

= 11 x 10^7 bacteriophages/mL.