Translate 53 Frame 1 N A G S R G G A S G G G R E L G G R R Q

Translate

5\'3\' Frame 1
N A G S R G G A S G G G R E L G G R R Q H C F K L A K L T R A R G I L R S I P T R G H G T G P C F P L L G P R E P G S A F C R V P R P P L Q G R A D P T R W R F Met E N F Q K V E K I G E G T Y G V V Y K A R N K L T G E V V A L K K I R L D T Stop V A S V P G T P N W G P P Stop L S P P T P H G R V A V Q G P E E S R E G L L Stop K W R G G L G A S R R Stop R V Y L Y S L G R V Stop G G V E S Met E N F L P K L S R I V P P N V R L Q T R G E K G G L Stop D E V Q D S P W S G V Met W E P A R I A F L N E E P S S L P H P H L R I L S S F Q R Met A V E P H W P L W G G W G L L L H F F P S I T V S L L H L H Q A A L L T Y P W E K R R Stop W P Stop Y I Q K P H P D Y P R I S S Y H H P F S S L T F L G G A G W C L L G G K K Stop L G G G E R N I C N H I P I S A F P T S P S E T E G V P S T A I R E I S L L K E L N H P N I V K Stop V C V Stop E V I Q L E R R I S S V C T V W A F L S L T H L H F L K L S F S R L L D V I H T E N K L Y L V F E F L H Q D L K K F Met D A S A L T G I P L P L I K V Met L L I S S S H H G H V L G D W W Q A I Q G D I L Stop F W P P S E P S S P I H T H S P S F C V S F P A H Y I H Stop P Stop G W T E Q S K L K L Stop Stop V N L A T Q V G G Q Met K L R Stop T G F E S T W Stop I P P K S P S I W W K T Stop L V S P Y C L F Stop G W I L H S Q S Y F Q S I N K H F F N A Q D V E K G W K I E Stop D L V L I L S G L T V H W E K Stop L V N Met Stop L Stop S N I I K A L V E F Stop G H R A N P V C S L Stop W R N T V L S F S F V R A I C S S C S R A Stop L S A I L I G S S T E T L N L R I C L L T Q R G P S S Stop Q T L D Stop P E L L E S L F V L T P Met R Stop V P L C L F S L S F P R G V N Stop G I H K V T K N I W L T V S Y Stop V E I I S Stop H P K E S Stop G Met H G I H T V L L T G P T P V I P I L W E A E V G G S L E P R S S R P S W A T Stop R D P I S T N L Q K E K I Stop K Stop N Y D Q F V S S F S R K I S N Stop G F S P E L W T G N Q W E G K D D G G K E T A C Stop E A K S K Q S S V E E L R C G N F H T L Stop T T P P L P I P V P Q V V T L W Y R A P E I L L G C K Y Y S T A V D I W S L G C I F A E Met V W R L A Q V P P S P L P L L P T S K N N R T A S W P R P Met A L L S Q G S L S K V A P R G Met V G K D A T V A L I S T T V L G Y P Q T A L V P G I H L L S L K Stop V K A F L Q L F Stop L Stop S A Y I W E N D S I Stop C L F Y F R P S F Q G L Stop T L L Y G A S N V V K T T V V T L P T P F I Stop T A D L G R V Q W L T P I I P A L W E A E V G G S P E V R S L R P A W P T C Stop N P V S T K N T K I S Q V W L L G I T H L Stop S Q L L G R P R Q E N R L N P G D G G C S D Q D C T T A L Q P G R Q N E I P S Q K K K K K K K K K D L D H V P L Q P L A F Q T E G S L K P G F Met Stop K L P S P L I Stop Q Y S G A V G C L Q I S N Met F Y C L L S W L Stop P R A Stop R P L A Y T Y V Met C I Y P P S A L P Y N C P Y S S L N S S K N I C Stop A P S G Y P A P C Stop V L W G T Q K K W K T E S L P A V L V S R S G C I T R L G D D R S V Y P L P R E W L G I P L L H V S G T P D I H P L P D P P S L G D L Q S H G W G K E G G G E E T D E G T S L S Q V L C D Stop P H E R P W G G S H G A L L T F Y C L W E L L C I E E S F D Stop R Q R G S W Y F L F P H F Q V T R R A L F P G D S E I D Q L F R I F R T L G T P D E V V W P G V T S Met P D Y K P S F P K W A R Q D F S K V V P P L D E D G R S L L S V R V G T C F P S F I S P R E G L F Q D E G R Met R P Stop N L G L S V S F P W F L L S L L A H Stop F S Y G R Met E V R I L P W V E G V L V S Stop F L G T P A A H L V H Y H I I E V N Met H L S L Stop Q Met L H Y D P N K R I S A K A A L A H P F F Q D V T K P V P H L R L Stop Stop P S Stop S P Q P Stop S H P L L Q C G L D Q A W P W A I W T Q V G P L N L P Stop T L T F Stop S W P A N S G N T G V K G G N Q Stop K Stop K E V S V L D A L K L A S T T L S P F S Stop L L L K R V G I K I I L K K P S Y T L D L P Y Q S L N A P Stop L L F P V F G Met T R I P S L L L P Q C L Stop R P N D S G G Stop V G A F E N Q V K Q N H W E E S I L K N S V E K I D P I S L Y P S Stop C F A S P N R L G D Stop R L S P G G A A E K Stop L A P V P L F V P S T G Met R N L G G P E T G I V L H S N L L L H H G L Met R Q V R D V Stop I F L F L L V F L V V Q L P R I P D P I F L Stop R P P P T P Stop E L E V R V Stop A S F Stop E C Stop H F F R A V I E Stop G H G Stop K Y F F K R R Met N N Y I Y I S G Y I Q Stop Stop S W L F F F F F G H S G W I C C H V H L G V L Stop Stop Q C Stop K K K A F F F Y D L S L S P L S L S A L A I N V I C N L V C S S L K K C A Stop F Y

5\'3\' Frame 2
T R E A G A G P L V A V G N S V G G G N I V S S W P N Stop Q E R E V Y C V P S R P G A T V L G P V S P S S A P E S Q G P P S A G F P G P R S R A G L T R L A G A S W R T S K R W K R S E R A R T E L C T K P E T S Stop R E R W W R L R K S A W T R E W P L Y P G L L T G D L L D C P P Q P P T G G Stop P S R D R K R A G R D F F R S G E V G W G P V E G E E Y T Y T P W G E Y R V V W N P W K T F F P N Stop A G S C P Q Met C D Y R L G E R K E V S E Met R S K T L H G V E L C G N R R E S P F Stop Met K S P L H C P T L T L E F S P L S K E W Q L N L T G P S G E A G G Y S C I F S P P L Q S P C F T F T R R L Y L P T P G K R G D N G L N I S K N H T L T T Q E L A L T I T L S L L S L S Stop G V L G G V S L G E R N D Stop V G G K G I F V T I F P S L L S Q P L Q V R L R V C P V L P S E R S L C L R S L T I L I L S S K Y A S E R Stop S S W K G G Stop V L S V Q C G H F S L S H T S I S S N F P S L G C W Met S F T Q K I N S T W F L N F C T K I S R N S W Met P L L S L A F L F P S S R Stop C F S S A P L I Met G Met S W G T G G R Q F R V I F Y D F G L L L S P H L L Y T H T P L L F V S P S L L I I F I N P R V G L N N Q S Stop N S S E S T Stop Q L R W E V R Stop N S D K R D L R A L G K F L Q K A L P F G G R P S Stop Stop V P I V Y F R A G F F T P R A T F N L L T N I F S Met H R Met Stop K R D G K L S K T W S L S S L G Stop Q S I G R N S L Stop I C N Y N P T Stop Stop R L Stop Stop S F R G T E Q T Q S A H C N G E T Q S S L S P L S E L S V P A A P G P S F L P F S S G P P P R P Stop T S E S A Y Stop H R G G H Q A S R L W T S Q S F W S P C S Y L H P Stop G E S L Y V F F L Stop A S Q E V L T R V F T K L L K I S G Stop Q F L T R Stop K Stop S L D I L K S L R V C Met E F I L C C Stop L G P H L Stop Y Q Y F G R L R W E D H L S P G V R D H H G Q H S E T P S L Q I Y K K K K F R N K I Met T N L S Q V F P G R S Q I R G S V Q N Y G L E I S G R G K Met Met E G R K L L V K R P R V S R V V L R N Stop D A G I S I P Y K P P R P S L F P S L R W Stop P C G T E L L K S S W A A N I I P Q L W T S G A W A A S L L R W Y G G L P K F H P A P S L S S P H P R T T E L L L G P D L W P F Y H R V L S L K Stop H Q G E W W E R Met Q L L P Stop Y Q P Q C Stop D I L K Q P Stop Y L V Y I S Y P Stop N K L K H F C S C F S C S L H I F G R Met I P F S A S F I S G L H F K A C R P C C Met V P A Met Stop Stop R Q L W S L Y P H L S F K L Q I Stop A G C S G S H L Stop Y Q H F G R L R Stop V D H L R S G V Stop D Q P G Q H V K T L S L L K I Q K L A R C G Y L G L H T C N P S Y L G G Q G R R I G Stop T R E Met E V A V T K I A P L H S S L G D R Met R F H L K K K K K K K K K K I Stop I Met F P F N L W L F R L K D P Stop S L A L C R S S H L L Stop Y N S T V V Q Stop A V F K S A I C F I V F Y L G C N Q E L K D H Stop P I H Met Stop C A F I P P V H Y L T I V R I P L S I H Q K I F V K H L V G T Q H H A R C C G E H R R N G R Q S L C P L C S Y L E V A A S Q G W G Met T A V S T P Y P V S G L G Y L C Y Met S V A P Q T F T P S Q T H P A L G I C K A Met V G G R K E G A R R Q Met K E L H C L R F C V T D P Met K G P G E G V Met G P C Stop P F T V C G N S F V Stop R R V L T D V N V G L G I S S F P I F R Stop L A G P Y S L E I L R L T S S S G S F G L W G P Q Met R W C G Q E L L L C L I T S Q V S P S G P G K I L V K L Y L P W Met K Met D G A C Y R Stop E W A P V F P H S F L P G K G F S R Met K E G Stop D P E I W A S V F H F P G S C S P C W H T D S A Met G G W K Stop E F C L G Stop K E F W F P D F W E H L L P I Stop S T I T S L K S T C I S P S S K C C T T T L T S G F R P R Q P W L T L S S R Met Stop P S Q Y P I F D S D S L L E A P S P N L T L S S S V G L T R L G L G L F G L R W A L Stop T C L K H S P S S L G Q P T L G I Q G Stop K G G T S E N E R K F Q Y Stop Met H L S Stop P P P P F P P S L S Y C Stop R G L V Stop K Stop F Stop K S L P T R Stop I C R T N L Stop Met P H N Y Y F Q C L G Stop P G S Q A S C C H N V Y K G Q Met I A G A K L V L L R T K Stop N K T T G R S L F Stop R I R L K K Stop I Q S V Y T L V S V L P H L I G W E T E D S A R V G L Q K N D W P Q S P C L S L L Q A Stop G I W E A L R Q G L C F I P I Y C F T Met A L Stop G R Stop E Met F E F F S S F Stop Y S Stop L F S C Q G S L I P F S S D V H L L P H R S Stop K L G F R H H F E N A D T F S G L Stop L S E G Met G K N I S L K E G Stop T I I F I F Q V I S N S R V G F F F F F L V I V G G F V A Met C T L G F C N D S A K K K K H F F F Met I C L C H P C P Stop V L L L L T L F V I Stop F V A H Stop K N V P S F I

5\'3\' Frame 3
R G K Q G R G L W W R S G T R W E A A T L F Q V G Q I D K S E R Y T A F H P D P G P R Y W A L F P P P R P P R A R V R L L Q G S Q A P A P G P G Stop P D S L A L H G E L P K G G K D R R G H V R S C V Q S Q K Q V D G R G G G A Stop E N P P G H V S G L C T R D S Stop L G T S L I V P P N P P R A G S R P G T G R E Q G G T S L E V E R W V G G Q Stop K V K S I L I L P G E S I G W C G I H G K L S S Q T E P D R A P K C A T T D S G R E R R S L R Stop G P R L S Met E W S Y V G T G E N R L S E Stop R A L F T A P P S P Stop N S L L F P K N G S Stop T S L A P L G R L G A T P A F F P L H Y S L P A S P S P G G F T Y L P L G K E E I Met A L I Y P K T T P Stop L P K N Stop L L P S P F L F S H F P R G C W V V S P W G K E Met T R W G G K E Y L Stop P Y S H L C F P N L S K Stop D Stop G C A Q Y C H P R D L S A Stop G A Stop P S Stop Y C Q V S Met R L R G D P A G K E D K F C L Y S V G I S L S H T P P F P Q T F L L Stop A A G C H S H R K Stop T L P G F Stop I S A P R S Q E I H G C L C S H W H S S S P H Q G N A S H Q L L S S W A C L G G L V A G N S G Stop Y F Met I L A S F Stop A L I S Y T H T L P F F L C L L P C S L Y S L T L G L D Stop T I K V E T L V S Q P S N S G G R S D E T Q I N G I Stop E H L V N S S K K P F H L V E D L A S E S L L S I L G L D S S L P E L L S I Y Stop Q T F F Q C T G C R K G Met E N Stop V R L G P Y P L W A D S P L G E I A C K Y V T I I Q H N K G F S R V L G A Q S K P S L L T V Met E K H S P L F L L C Q S Y L F Q L L Q G L A F C H S H R V L H R D L K P Q N L L I N T E G A I K L A D F G L A R A F G V P V R T Y T H E V S P F Met S F F S E L P K R C Stop L G Y S Q S Y Stop K Y L A N S F L L G R N N L L T S Stop R V L G Y A W N S Y C V A N W A H T C N T N T L G G Stop G G R I T Stop A Q E F E T I Met G N I A R P H L Y K S T K R K N L E I K L Stop P I C L K F F Q E D L K L G V Q S R T Met D W K S V G G E R Stop W R E G N C L L R G Q E Stop A E Stop C Stop G T E Met R E F P Y P I N H P A P P Y S R P S G G D P V V P S S Stop N P P G L Q I L F H S C G H L E P G L H L C Stop D G Met E A C P S S T Q P P P S P P H I Q E Q Q N C F L A Q T Y G P S I T G F S L Stop S S T K G N G G K G C N C C P D I N H S V R I S S N S L S T W Y T S L I P E I S Stop S I S A A V L A V V C I Y L G E Stop F H L V P L L F Q A F I S R L V D L V V W C Q Q C S E D N C G H F T H T F H L N C R F R Q G A V A H T Y N T S T L G G Stop G R W I T Stop G Q E F E T S L A N Met L K P C L Y Stop K Y K N Stop P G V A T W D Y T P V I P A T W E A K A G E S V E P G R W R L Q Stop P R L H H C T P A W A T E Stop D S I S K K K K K K K K K R F R S C S P S T S G F S D Stop R I L E A W L Y V E A P I S F N I T V Q W C S R L S S N Q Q Y V L L S F I L V V T K S L K T I S L Y I C N V H L S P Q C I T L Q L S V F L S Q F I K K Y L L S T Stop W V P S T Met L G A V G N T E E Met E D R V S A R C A R I Stop K W L H H K V G G Stop P Q C L P P T P Stop V A W D T F A T C Q W H P R H S P P P R P T Q P W G S A K P W L G E G R R G R G D R Stop R N F I V S G S V Stop L T P Stop K A L G R E S W G P A D L L L S V G T P L Y R G E F Stop L T S T W V L V F P L S P F S G D S P G P I P W R F Stop D Stop P A L P D L S D S G D P R Stop G G V A R S Y F Y A Stop L Q A K F P Q V G P A R F Stop Stop S C T S P G Stop R W T E L V I G E S G H L F S L I H F S Q G R A F P G Stop R K D E T L K S G P Q C F I S L V P A L P V G T L I Q L W E D G S E N S A L G R R S S G F L I S G N T C C P F S P L S H H Stop S Q H A S L P L A N A A L R P Stop Q A D F G Q G S P G S P F L P G C D Q A S T P S S T L I A F L K P P A L I S P S P P V W A Stop P G L A L G Y L D S G G P S E L A L N T H L L V L A S Q L W E Y R G E R G E P V K Met K G S F S I R C T Stop V S L H H P F P L L L V I A E E G W Y K N N F K K A F L H V R F A V P I S E C P I I I I S S V W D D Q D P K P P A A T Met F I K A K Stop Stop R G L S W C F Stop E P S K T K P L G G V Y F K E F G Stop K N R S N Q F I P Stop L V F C L T Stop Stop A G R L K T Q P G W G C R K Met I G P S P L V C P F Y R H E E S G R P Stop D R D C A S F Q S I A S P W P Y E A G E R C L N F S L P F S I L S C S V A K D P Stop S H F P L T S T S Y P I G V R S Stop G L G I I L R Met L T L F Q G C D Stop V R A W V K I F L Stop K K D E Q L Y L Y F R L Y P I V E L A F F F F F W S Stop W V D L L P C A P W G F V Met T V L K K K S I F F L Stop F V S V T L V L E C S C Y Stop R Y L Stop F S L Stop L I K K Met C L V L

3\'5\' Frame 1
Y K T R H I F L Met S Y K L N Y K Stop R Stop Stop Q E H S R T R V T E T N H K K K Met L F F F S T V I T K P Q G A H G N K S T H Y D Q K K K K K A N S T I G Y N L K Y K Y N C S S F F Stop R N I F T H A L T Q S Q P Stop K S V S I L K Met Met P K P Stop L L T P Met G Stop E V D V R G K W D Q G S L A T E Q L R I L K G R E K F K H L S P A S Stop G H G E A I D W N E A Q S L S Q G L P D S S C L Stop K G Q T R G L G P I I F L Q P H P G Stop V F S L P A Y Stop V R Q N T N Stop G I N Stop L D L F F Q P N S L K Stop T P P S G F V L L G S Q K H Q L S P R Y H L A F I N I V A A G G L G S W S S Q T L E I I I Met G H S E I G T A N L T C R K A F L K L F L Y Q P S S A I T K R R G K G W W R L T Stop V H L I L K L P F I F T G S P L S P L Y S Q S W L A K T R R Stop V F K A S S E G P P E S K Stop P K A K P G Q A H T G G E G E I R A G G F K K A I R V E D G V L A W S H P G R K G E P G L P W P K S A C Stop G R S A A F A R G R D A C Stop L Q Stop C D S G L N G Q Q V F P E I R K P E L L L P K A E F S L P S S H S Stop I S V P T G R A G T R E Met K H Stop G P D F R V S S F L H P G K A L P W E K Stop Met R E N R C P L S P I T S S V H L H P G E V Q L Y Stop N L A G P T W G N L A C N Q A Stop K Stop L L A T P P H L G S P E S E R S G R A G Q S Q N L Q G I G P G E S P E N G E R G N T K T H V D V S Q N S P L Y K G V P T D S K R S A G P H D S L P R A F H G V S H T E P E T Met K F L H L S P R P L L P S P N H G F A D P Q G W V G L G G G E C L G C H Stop H V A K V S Q A T H G V G G R H C G H P P T L Stop C S H F Stop I R A Q R A E T L S S I S S V F P T A P S Met V L G T H Stop V L N K Y F L Met N Stop E R N T D N C K V Met H W G D K C T L H Met Y R L Met V F K L L V T T K I K D N K T Y C Stop F E D S L L H H C T V I L K E Met G A S T Stop S Q A S R I L Q S E K P E V E G E H D L N L F F F F F F F F F E Met E S H S V A Q A G V Q W C N L G H C N L H L P G S T D S P A L A S Q V A G I T G V Stop S Q V A T P G Stop F L Y F Stop Stop R Q G F N Met L A R L V S N S Stop P Q V I H L P Q P P K V L V L Stop V Stop A T A P C L N L Q F K Stop K V W V K Stop P Q L S S L H C W H H T T R S T S L E Met K A Stop N K R G T K W N H S P K Y Met Q T T A K T A A E Met L Stop L I S G I R D V Y Q V L R L F E D I L T L W L I S G Q Q L H P F P P F P L V L L Stop R E N P V I E G P Stop V W A K K Q F C C S W Met W G G E G G G W V E L G Q A S I P S Q Q R C S P G S R C P Q L W N N I C S P G G F Q E L G T T G S P P E G R E Stop G G A G W F I G Y G N S R I S V P Q H Y S A Y S W P L N K Q F P S L H H L S P P T D F Q S I V L D Stop T P N L R S S W K N L R Q I G H N F I S K F F L F V D L Stop R W G L A Met L P Met Met V S N S W A Q V I L P P Q P P K V L V L Q V W A Q L A T Q Y E F H A Y P K T L Stop D V K R L F L P S K K L L A R Y F Stop Stop L C E Y P S Stop H L L G S S E K K D I K G L T S W V Stop V R T G T P K A L A S P K S A S L Met A P S V L I S R F Stop G L R S R W R T R Stop E W Q K A R P W S S W N R Stop L Stop Q R R K R G L C F S I T V S R L G L L C A P K T L L K P L L C W I I V T Y L Q A I S P N G L S A Q R G Stop G P S L T Q F S I P F L H P V H Stop K N V C Stop Stop I E S S S G S E E S S P K I D N R D S L A R S S T K W K G F L E E F T K C S Q I P F I Stop V S S D L P P E L L G Stop L T R V S T L I V Q S N P R V N E Y N E Q G R R H K K K G S V C V Stop E Met R A Q K E A K I I K Y H P E L P A T S P P R H A H D E R S Stop Stop E A L P Stop Stop G E E E C Q Stop E Q R H P Stop I S Stop D L G A E I Q K P G R V Y F L C E Stop H P A A Stop R R K V Stop G N G G V Stop E R E Met P T L Y R Q N L S S F P A G S P L R R I L T Stop Q Y Stop D G Stop A P Stop A E R S L G W Q Y W A H P Q S H L E R L G K Q R W E Y G Y K Y S F P P H L V I S F P Q G D T T Q H P L G K Stop E K R K G D G K S Stop F L G S Q G V V F G Y I K A I I S S F P R G R Stop V K P P G E G E A G R L Stop W R G K N A G V A P S L P R G A S E V Q L P F F G K R R E F Stop G E G G A V K R A L H S E R R F S P V P T Stop L H S Met E S L G P H L R D L L S L P E S V V A H L G A R S G S V W E E S F P W I P H H P I L S P G S I S I L F T F Y W P P T H L S T S K E V P P C S L P V P G R L P A R G G L G G T I K E V P S Stop E S R V Q R P L T C P G G F S Stop A P P P L P S T C F W L C T Q L R T C P L R S F P P F G S S P Stop S A S E S G Q P G P G A G A W E P C R R R T L A L G G R G G G N R A Q Y R G P G S G W N A V Y L S L L S I W P T Stop N N V A A S H R V P D R H Q R P R P C F P R

3\'5\' Frame 2
I K L G T F F Stop Stop A T N Stop I T N N V N S K S T Q G Q G Stop Q R Q I I K K K C F F F L A L S L Q N P K V H Met A T N P P T Met T K K K K K K P T L L L D I T Stop N I N I I V H P S F K E I F L P Met P S L N H S P E K V S A F S K Stop C L N P N F Stop L L W G R R W T S E E N G I R D P W Q L N N Stop E Y Stop K E E K N S N I S H L P H K A Met V K Q Stop I G Met K H N P C L R A S Q I P H A C R R D K Q G D W G Q S F F C S P T R A E S S V S Q P I R Stop G K T L T R V Stop T D W I Y F F N R I L Stop N R L L P V V L F Y L V L K S T N L A P A I I W P L Stop T L W Q Q E A W D P G H P K H W K Stop Stop L W G I Q R L V R Q I Stop R V G R L F Stop N Y F Y T N P L Q Q Stop L R E G G K G G G G Stop L K C I Stop Y Stop N F L S F S L V P P F H P C I P R V G W P R L E G E C L R Q V Q R A H L S P N S P R P S L V K P T L E E R V R L G L G A S R R L S E S K Met G Y W L G H I L E E R V S Q G C L G R N P L V R V V V Q H L L E G E Met H V D F N D V I V D Stop Met G S R C S Q K S G N Q N S F Y P R Q N S H F H P P I A E S V C Q Q G E Q E P G K Stop N T E A Q I S G S H P S F I L E K P F P G R N E Stop G K T G A H S H R Stop Q A P S I F I Q G R Y N F T K I L P G P L G E T W L V I R H R S N S W P H H L I W G P Q S P K D P E E L V N L R I S R E Stop G P A S H L K Met G K E E I P R P T L T S V K T L L Y T K E F P Q T V K G Q Q G P Met T P S P G P F Met G S V T Q N L R Q Stop S S F I C L L A P S F L P P T Met A L Q I P K A G W V W E G V N V W G A T D Met Stop Q R Y P K P L T G Stop G V D T A V I P Q P C D A A T S R Y E H S G Q R L C L P F L L C S P Q H L A W C W V P T R C L T N I F Stop Stop I E R G I R T I V R Stop C T G G I N A H Y I C I G Stop W S L S S W L Q P R Stop K T I K H I A D L K T A Y C T T V L L Y Stop R R W E L L H K A R L Q G S F S L K S Q R L K G N Met I Stop I F F F F F F F F F L R W N L I L S P R L E C S G A I L V T A T S I S R V Q P I L L P W P P K Stop L G L Q V C N P K Stop P H L A N F C I F S R D R V L T C W P G W S Q T P D L R Stop S T Y L S L P K C W Y Y R C E P L H P A Stop I C S L N E R C G Stop S D H S C L H Y I A G T I Q Q G L Q A L K Stop R P E I K E A L N G I I L P N I C R L Q L K Q L Q K C F N L F Q G Stop E Met Y T R Y Stop G C L R I S Stop H C G Stop Y Q G N S C I L S H H S P W C Y F R E R T L Stop Stop K G H R S G P R S S S V V L G C G E E R E G A G W N L G K P P Y H L S K D A A Q A P D V H S C G I I F A A Q E D F R S S V P Q G H H L R D G N R E G R G G L Stop G Met E I P A S Q F L N T T L L T L G L L T S S F L P S I I F P L P L I S S P Stop F W T E P L I Stop D L P G K T Stop D K L V I I L F L N F F F L Stop I C R D G V S L C C P Stop W S R T P G L K Stop S S H L S L P K Y W Y Y R C G P S Stop Q H S Met N S Met H T L R L F R Met S R D Y F Y L V R N C Stop P D I F S N F V N T L V N T S W E A Q R K K T Stop R D S P H G C K Y E Q G L Q K L W L V Q S L L A Stop W P P L C Stop Stop A D S E V Stop G L G G G P D E N G R K L G P G A A G T D S S D K G E R E D C V S P L Q Stop A D W V C S V P L K L Y Stop S L Y Y V G L Stop L H I Y K L F L P Met D C Q P R E D K D Q V L L N F P S L F Y I L C I E K Met F V N R L K V A L G V K N P A L K Stop T I G T H Stop L G L P P N G R A F W R N L P S A L K S R L S E F H L T S H L S C Stop V D S L E F Q L Stop L F S P T L G L Met N I Met S R E G D T K R R G V C V Y R R Stop G L R R R P K S Stop N I T L N C L P P V P Q D Met P Met Met R G A D E K H Y L D E G K R N A S E S R G I H E F L E I L V Q K F K N Q V E F I F C V N D I Q Q P R E G K F E E Met E V C E R E K C P H C T D R T Y P P F Q L D H L S D A Y L L D N I R Met V K L L K Q R D L S D G S T G H T L S L T W R G W E S R D G N Met V T N I P F P P T Stop S F L S P K E T P P S T P Stop E S E R R E R V Met V R A N S W V V R V W F L D I L R P L S P L F P G V G K Stop S R L V K V K Q G D C N G G E K Met Q E Stop P P A S P E G P V R F N C H S L E R G E N S K V R V G Q Stop R G L F I Q K G D S R R F P H N S T P W R V L D L I S E T S F L S P S L Stop S H I W G H D P A Q F G K K V F H G F H T T L Y S P Q G V Stop V Y S S P S T G P Q P T S P L L K K S L P A L F R S L D G Y P P V G G W G G Q S R R S P V R S P G Y R G H S R V Q A D F L K R H H L S R Q L V S G F V H N S V R A L S D L F H L L E V L H E A P A S R V S P A L E R G P G N P A E G G P W L S G A E E G E T G P S T V A P G R D G T Q Y T S R S C Q F G Q L E T Met L P P P T E F P T A T R G P A P A S R V

3\'5\' Frame 3
Stop N Stop A H F F N E L Q T K L Q I T L I A R A L K D K G D R D K S Stop K K N A F F F Stop H C H Y K T P R C T W Q Q I H P L Stop P K K K K K S Q L Y Y W I Stop P E I Stop I Stop L F I L L L K K Y F Y P C P H S I T A L K K C Q H S Q N D A Stop T L T S N S Y G V G G G R Q R K Met G S G I L G N Stop T T K N T K R K R K I Q T S L T C L I R P W Stop S N R L E Stop S T I P V S G P P R F L Met P V E G T N K G T G A N H F S A A P P G L S L Q S P S L L G E A K H Stop L G Y K L I G S I F S T E F F K I D S S Q W F C F T W F S K A P T Stop P P L S F G L Y K H C G S R R L G I L V I P N T G N N N Y G A F R D W Y G K S N V Stop E G F F K I I F I P T L F S N N Stop E K G E R V V E A N L S A S N T E T S F H F H W F P P F T P V F P E L A G Q D Stop K V S V Stop G K F R G P T Stop V Q I A Q G Q A W S S P H W R R G Stop D Stop G W G L Q E G Y Q S R R W G T G L V T S W K K G Stop A R A A L A E I R L L G S Stop C S I C Stop R E R C Met L T S Met Met Stop Stop W T K W A A G V P R N Q E T R T P S T Q G R I L T S I L P Stop L N Q C A N R E S R N Q G N E T L R P R F Q G L I L P S S W K S P S L G E Met N E G K Q V P T L T D N K L R P S S S R G G T T L L K S C R A H L G K L G L Stop S G I E V T P G H T T S S G V P R V R K I R K S W S I S E S P G N R A R R V T Stop K W G K R K Y Q D P R Stop R Q S K L S S I Q R S S H R Q Stop K V S R A P Stop L P P Q G L S W G Q S H R T Stop D N E V P S S V S S P P P S F P Q P W L C R S P R L G G S G R G Stop Met S G V P L T C S K G I P S H S R G R G Stop T L R S S P N L V Met Q P L L D T S T A G R D S V F H F F C V P H S T Stop H G A G Y P L G A Stop Q I F F D E L R E E Y G Q L Stop G N A L G G Stop Met H I T Y V Stop A N G L Stop A L G Y N Q D K R Q Stop N I L L I Stop R Q P T A P L Y C Y I K G D G S F Y I K P G F K D P S V Stop K A R G Stop R G T Stop S K S F F F F F F F F F Stop D G I S F C R P G W S A V V Q S W S L Q P P S P G F N R F S C L G L P S S W D Y R C V I P S S H T W L I F V F L V E T G F Stop H V G Q A G L K L L T S G D P P T S A S Q S A G I I G V S H C T L P K S A V Stop Met K G V G K V T T V V F T T L L A P Y N K V Y K P Stop N E G L K Stop K R H Stop Met E S F S Q I Y A D Y S Stop N S C R N A L T Y F R D K R C I P G T K A V Stop G Y P N T V V D I R A T V A S F P T I P L G A T L E R E P C D R R A I G L G Q E A V L L F L D V G R R G R G L G G T W A S L H T I S A K Met Q P R L Q Met S T A V E Stop Y L Q P R R I S G A R Y H R V T T Stop G T G I G R G G V V Y R V W K F P H L S S S T L L C L L L A S Stop Q A V S F P P S S F P S H Stop F P V H S S G L N P Stop F E I F L E K L E T N W S Stop F Y F Stop I F S F C R F V E Met G S R Y V A H D G L E L L G S S D P P T S A S Q S I G I T G V G P V S N T V Stop I P C I P Stop D S L G C Q E I I S T Stop Stop E T V S Q I F L V T L Stop I P Stop L T P L G K L R E K R H K G T H L Met G V S T N R D S K S S G Stop S K V C Stop L D G P L C V N K Q I L R F K V S V E D P Met R Met A E S Stop A L E Q L E Q I A L T K E K E R T V F L H Y S E Q T G F A L C P Stop N S T K A F I Met L D Y S Y I F T S Y F S Q W T V S P E R I R T K S Y S I F H P F S T S C A L K K C L L I D Stop K Stop L W E Stop R I Q P Stop N R Q Stop G L T S Stop V F H Q Met E G L F G G I Y Q V L S N P V Y L S F I Stop P P T Stop V A R L T H Stop S F N F D C S V Q P Stop G Stop Stop I Stop Stop A G K E T Q K E G E C V C I G D E G S E G G Q N H K I S P Stop I A C H Q S P K T C P Stop Stop E E L Met R S I T L Met R G R G Met P V R A E A S Met N F L R S W C R N S K T R Stop S L F S V Stop Met T S S S L E K E S L R K W R C V R E R N A H T V Q T E L I L L S S W I T S Q T H T Y L T I L G W L S S L S R E I S R Met A V L G T P S V S L G E V G K A E Met G I W L Q I F L S P P P S H F F P P R R H H P A P P R K V R E E K G Stop W Stop E L I L G Stop S G C G F W I Y Stop G H Y L L F S Q G Stop V S K A A W Stop R Stop S R E T V Met E G K K C R S S P Q P P Q R G Q Stop G S T A I L W K E E R I L R Stop G W G S E E G S S F R K A I L A G S H I T P L H G E S W T S S Q R P P F S P R V C S R T F G G T I R L S L G R K F S Met D S T P P Y T L P R E Y K Y T L H L L L A P N P P L H F Stop R S P S L L S S G P W T A T R P W G V G G D N Q G G P Q L G V P G T E A T H V S R R I F L S A T T S P V N L F L A L Y T T P Y V P S P I F S T F W K F S Met K R Q R V G S A R P W S G G L G T L Q K A D P G S R G P R R G K Q G P V P W P R V G Met E R S I P L A L V N L A N L K Q C C R L P P S S R P P P E A P P L L P A

Use the CCDS sequence and the site http://web.expasy.org/translate/ to translate
the DNA sequence to the encoded amino acid.---- I already did the translation and have added the Frames,the different frames are lsited please choose the one that you beileve is most approporaite and explain the reasoning which is question part a.
a. The program gives back several different options (it says please select one of the
following frames). Which one do you select? Explain.

Solution

DNA is translated in to protein sequence in form of non overlapping triplets called codons. The translation happens only from 5` end to 3` end and complement 3`- 5`end. As the sequence starts with three letters there can be three combinations of codon reading can be done from 5` end to 3` end. Out of six ways of reading one frame is biologically relevant frame and called open reading frame. Divide the sequence into 6 different reading frames(+1, +2, +3, -1, -2 and -3). of the six frames select the frames that have a codon AUG called start codon and ends with a stop codon.

Below is the longest sequence of ORF deduced from the protein sequence above coming from the frame 1 starting from the 229 nucletide to 1029 nucleotide where a open readinf frame from a start and stop codon can be generated. its translates to a 226 aminoacid sequence of bovine b2 glycoprotein.

MPRVCPFAGILENGTVRYTTFEYPNTISFSCHTGFYLKGA
SSAKCTEEGKWSPDLPVCAPITCPPPPIPKFASLSVYKPL
AGNNSFYGSKAVFKCLPHHAMFGNDTVTCTEHGNWTQLPE
CREVRCPFPSRPDNGFVNHPANPVLYYKDTATFGCHETYS
LDGPEEVECSKFGNWSAQPSCKASCKLSIKRATVIYEGER
VAIQNKFKNGMLHGQKVSFFCKHKEKKCSYTEDAQCIDGT
IEIPKCFKEHSSLAFWKTDASDVKPC