You are investigating a new bacterial operon that contains f

You are investigating a new bacterial operon that contains five regions (A, B, C, D and E) involved in coordinated regulation of transcription. One is the gene for a regulatory protein, one is the binding site for the regulatory protein, two produce structural enzymes (enzyme 1 and enzyme 2), and one is the promoter for the two enzyme genes.

The table below shows data collected for five different bacterial strains. A \"+\" indicates that the structural enzyme is produced and a \"-\" indicates that the enzyme is not produced. The \"signal\" is a molecule that either represses or induces the operon. Use the information to determine how the operon is regulated, and the identity of each region.

Part B

Parts of an operon that control the expression of both enzyme genes are called \"regulatory regions.\" The genes that produce the enzymes are called \"structural genes.\" Using the data in the table, determine which regions are regulatory regions and which are structural genes.

Sort each region into the correct category.

Regions: A, B, C, D, E

Regulatory Region(s) Structural Gene(s)

Solution

The operon contain 2 structural genes and 3 regulatory genes. A & C are structural genes and B, D & E are Regulatory genes. A gives Enzyme-1, C gives Enzyme-2; B gives regulatory region, D gives Regulator binding region and E gives Promoter region.