1112 AM 18 LO Done Practical 141 5 have R2 value plot axes a

11:12 AM 18% LO, Done Practical 14.1 (5) have R2 value plot axes and e Absorbance vs Concentration (1/M) 39028-00 14 1,21 bsorbance 06 04 0.2 000001 000002 0,00003 00000 0s 3) Note that A --Cl has the form of a straight line (that is, y ax+b). What does the slope of the standard curve correspond to? Where does your standard curve intercept the Y axis? t the intercept deviates from zero, explain why

Solution

From the graph, the equation for the graph is y=390.26x-0.0003

Comparing this with the equation of the straight line that is y=mx+c. The slope of the graph will be 390.26 and intercept is -0.0003.

Yes, the intercept deviates from zero. The intercept indicates the absorbance baseline when the concentration of the sample is zero or no sample is present. The absorbance is due to the reagent, it can be positive or negative.