Match the following descriptions with dyes A Counter stain f

Match the following descriptions with dyes A. Counter stain for acid fast stain B, Counter stain for Gram stain C. Primary stain for endospore D. Primary stain for acid fast stain E. Primary stain for Gram stain Carbol fuchsin Crystal violet Methylene blue Malachite green Safranin Select right match for the terms below. (Questions 25 through 29) A. Pink (with reference to Gram stain result) B. Blue C. Purple D. Red (with reference to Acid Fast stain result) Color of Staphylococcus aureus after acid fast stain Color of Bacillus megaterium after Gram stain Color of Pseudomonas aeruginosa after Gram stain Color of Pseudomonas aeruginosa after acid fast stain Color of Mycobacterium smegmatis after acid fast stain. Listeriosis is frequently be caused by eating food such as cheese or Listeria monocytogenes while stored in the refrigerator. Based on this monocytogenes may be described as A. a mesophile B. a psychrophile C. a psychrotroph D. a thermophile E. acid fast The GasPak anaerobic jar is used to A. create an oxygen-free environment for anaerobic growth B. provide adequate nutrients for anaerobic growth C. create selective media for anaerobic growth D. All the above are correct

Solution

A. Counter stain for acid fast stain is methylene blue used to differentiate acid fast and non acid fast bacteria.

B. Counter stain for gram staining is safranin used to differentiate for gram negative and gram positive.

C. Primary stain for endospore is malachite green.

D. Primary stain for acid fast staining is carbon fuchsin.

E. Primary stain of gram positive bacteria is crystal violet.

25. Colour of s. Aureus after acid fast stain is blue as it is non acid fast bacteria.

26. Colour of bacillus megaterium after gram stain is purple because it is gram positive bacteria.

27. Colour of pseudomonas aeruginosa after gram staining is pink as it is gram negative bacteria.

28. Colour of pseudomonas aeruginosa after acid fast stain is red colour

29. Colour of mycobacterium smegmatis after acid fast staining is red as it is acid fast bacteria.

30. Monocytogenes are the mesophile responsible for food poisoning ( image is cut so can\'t read complete sentences)

31. Answer is D i.e. all of above are correct for gas pack jar.