httpswwwyoutubecomwatchvBQyhG2VBI 3From video it said that t

https://www.youtube.com/watch?v=B_QyhG2-VBI.

3.From video, it said that the elute rate can speeded up by applying a pressure on the top of the column. Could you tell the problems which might be caused by such an operation?

4.Young wants to separate a phenol from a nature product with cholesterols by column chromatography. What your suggestion for him in both the stationary phase and eluent selection?

Solution

3. If you apply pressure on the top of a column, the elution rate increases and the separation of bands of different compounds decreases. If the pressure is too high , then the compounds from a mixture is difficult to separate.

Another problem is that: under high pressure the column can break.

4.

Stationary phase is silica gel

Eluent: 10:1 Hexane : Ethyl acetate

Phenol being more polar than cholesterol tends to retain in the column for longer time than cholesterol. So, Cholesterol will elute first and then phenol.