Contrast the specific and selectivity of glucose oxidase for

Contrast the specific and selectivity of glucose oxidase for beta-D glucose substrate vs. alcohol dehydrogenase for alcohols as substrates. Point out some key differences. Alcohol dehydrogenase Write a balanced equation for the reaction catalyzed by alcohol dehydrogenase using ethanol as a substrate.

Solution

1.

2. Equation of alcohol dehydrogenase using ethanol as a substrate:

CH₃CH₂OH + NAD⁺------------->CH3CHO + NADH + H⁺

Ethanol Acetyldehyde

Glucose Oxidase	Alcohol dehydrogenase
This is an oxido-reductase enzyme: Enzymes that catalyse the transfer of electrons.	This is an dehydrogenase enzyme: An enzyme that catalyses the removal of water.
It catalyses the oxidation of glucose to hydrogen peroxide and D-glucono lactone.	It aids in the interconversion of alcohols or aldehyde using NADP.
It specifically acts on beta-D-glucose than alpha-D-glucose. Only beta form is oxidized with this enzyme.	This enzyme is most active with ethanol, and its activity decreases as the size of alcohol increases.