You are working up a male genital culture You see no growth

You are working up a male genital culture. You see no growth on the blood agar plate but small colonies growing on the chocolate agar plate. The gram stain shows gram negative cocci in pairs. Which test will you do next?

indole

catalase

motility

oxidase

Solution

Oxidase test will be done next.

Because most probably the microorganism forming colony is Neisseria gonorrhoea. It is coccus, appears in pairs, coffee bean like. They show overgrowth in chocolate medium.

Oxidase test should be performed next, and carbohydrate glucose, maltose, sucrose test, in which it will oxidise only glucose.