You identify a new protein R2D2 by its similarity in sequenc

You identify a new protein R2D2 by its similarity in sequence to other proteins you suspect is a nuclear protein, state one experiment you would do to confirm that this is a nuclear protein.

Solution

The fact that a nuclear protein localizes in the nucleus can be used to confirm whether a newly identified protein is nuclear or not. In order to confirm it, the following immuno-cyto fluorescen or immuno-histo fluorescenceexperiment can be conducted:

Observation: Since the nuclear stain exclusively stains the nucleus, the nuclei of teh cell/tissue will look blue in color. Further, if the protein is nuclear in location, there would be observed a green signal as well. Upon merging the images of blue and green fields, the two colors would overlap each other. This confirms that the protein is of nuclear localization.