wno do we use chaperon protein for preparation of other prot

wno do we use chaperon protein for preparation of other proteins? 49 Write down the steps for protein purification and sequencing. 50. What is the difference between recombinant and engineering protein?

Solution

48) the protein has secondary, tertiary, quaternary structure.. I can perform it\'s function when it is correctly folded into its own tertiary and quaternary structure.. So, Molecular chaperons are those which helps in the proper folding of the newly formed protein into it tertiary an quaternary structure and helps in the protein function.. It also act as a heat shock protein and thus prevents the protein from and mechanical shock..!!

50) recombinant protein are those protein in which the gene sequence of a particular protein is inserted into the gene coding of another protein which results in the fusion of the those two protein and formation of one single new protein..!!

Wheraes in protein engineering, the protein itself are engineered to form a more new valuable protein products.. It is based on the engineering on protein structure, folding etc..!!

49) to sequence the protein we can go for Edman degradation, Sanger sequence, De Nova sequence by ESI MS/MS...

Purification includes, column chromatography, affinity chromatography, size exclusive chromatography, ultracentrifugation..!!