Q2 Allele 1 Probe TGG CCA T CCA TGG CCA 750bp 500bp 100bp Pr

Q2 Allele 1 Probe TGG CCA. T CCA. TGG CCA 750bp 500bp 100bp Probe TGG CCA TGA CCA TGG CCA Allele 2 800bp 600bp 400bp 200bp 100bp

Solution

Answer:

2. If the probe binds to allele 1, cleavage of the DNA with the restriction enzyme will show up a band at 400bp in the gel.

If the probe binds to allele 2, the restriction site at 500bp position will get abolished due to the presence of SNP. Thus, a band corresponding to 650bp will show up in the gel.

Homozygous allele 1: 3

Homozygous allele 2: 4

Heterozygous: 5

3. Individual 1: Homozygous allele 2

Individual 2: Homozygous allele 1

Individual 3: Heterozygous

Individual 4: Homozygous allele 2

Individual 5: Homozygous allele 1

4. Homozygous for allele 1: 5

Homozygous for allele 2: 3

Heterozygous: 1