1What would happen if you forgot to add dNTPs to PCR Assumin

1.What would happen if you forgot to add dNTPs to PCR? (Assuming all other ingredients are present)

2.Which of the following (is/are) not a reagent in PCR?

Solution

Question no-1 Answer no No reaction would occur.

because in PCR ,dNTP is crucial factor for polymerisation of primer . DAN polymerase bind to primer and template but lack of nucleotide DNA pol can not add nucleotide to primer. DNA polymerase have polymerase ,3-5 exonuclease activity if dNTP is not available in reaction then exonuclease activity perform by DNA polymerase result DNA polymerase eat primer also.

Question-2-Answer

Polymerase chain reaction require DNA polymerase for polymerization. Template DNA and Primer ,primer anneal to template DNA and its ready for polymerization.MgCl2, mg ion stabilized the phosphate group nad help in catalysation of reaction.

in given below matterial are not requir of PCR

Flourescent nucleotides- NUCLEOTIDE IS NOT REQUIRE FOR POLYMERISATION ,DEOXYNUCLEOTIDE CAN BE USE FOR PCR

Restriction enzymes- ITS CUT THE DNA at palidromic sequence

Agarose -requir to make gel to get seprated hte pcr products