Given a short discussion for each of the reagents of the pla

Given a short discussion for each of the reagents of the plasmid isolation procedure. Include the step the reagent is associated, and the function of the reagent (in relation to the DNA/ proteins/or other chemicals)* Note- Not all the reagents are provided for each buffer/ solution used in the Plasmid Isolation experiment. Include the resource(s) that you use to help answer this question.

1)200mM Sodium Hydroxide /1% SDS

Solution

1) 200mM Sodium hydroxide : It is used in alkaline lysis step of plasmid isolation.Sodium hydroxide helps to denature the genomic and plasmid DNA.It also denatures the cellular proteins that maintain the cell membrane structure.

2)1% SDS:It is also used in alkaline lysis step of plasmid isolation. SDS detergent causes cell lysis by solubilising the phospholipids and proteins of the cell membrane thereby releasing the contents of the cells.

Sources:

http://vlab.amrita.edu/?sub=3&brch=77&sim=314&cnt=1

https://www.gbiosciences.com/image/pdfs/protocol/BE-310_protocol.pdf