15 A researcher for GNC is experimenting with yeast extracts

15) A researcher for GNC is experimenting with yeast extracts to market them for their nutritious qualities. She prepares an extract of yeast cytoplasm and adds sugar to improve the taste. Unexpectedly, the extract begins to bubble a gas. The gas is

D. carbon dioxide from fermentation.

16) The researcher then decides to add some ATP and suddenly the bubbling stops. If she were to test for the presence of cellular metabolites, which of the following is likely to have accumulated in the yeast extract?

C. fructose-6-phosphate

15 is just there for context, can someone explain why the answer to 16 is fructose-6-phosphate?

Solution

WHEN GLUCOSE(SUGAR) FERMENTATION TAKE PLACE BY YEAST EXTRACT FOLLOWING REACTION S POSSIBLE

GLUCOSE+2ADP+2Pi-----------2 ETHANOL+2CO₂ +2ATP+2H₂O

IN ABOVE CO₂ IS PRODUCED VERY FAST AND CONTINUOUSLY DURING INTIAL PHASE OF REACTION THAT SLOW DOWN EVENTUALLY. AT THIS POINT IF ATP IS ADDED TO THE SOLUTION THEN FRUCTOSE 6 PHOSPHATE WILL GET PRODUCED. THIS ACCUMULATES BEACUASE IT IS PRESENT BETWEEN ENEGRY FORMATION AND ENERGY PAY OFF REACTIONS THAT ENDS THE PROCESS. THERE WILL BE BACKWARD EFLUX OF FRUCTOSE 6 PHOSPHATE TO STOP BUBBLING IN YEAST EXTRACT SOLUTION.