3 The following is part of a gene ATG Ace occ Mr CAA CTA TAT

3 The following is part of a gene ATG Ace occ Mr CAA CTA TAT TGA 3 3\' TAC roG ccG TTA GTT GAT ATA ACT 5\' eotid a) Determine the amino aci of the protein encoded by this sequence using the code provided on the next (1 p) genetic result from each of the Give the altered amino acid sequence of the protein following mutations (2 pts): b) A transition at nucleotide 11 c) A transition at nucleotide 13 e) An A T transversion at nucleotide 15

Solution

A)

The amino acid sequence will be

M T G N Q L Y

B)

Transition at 11 residues means Ato G conversion will result (N to S)

MT G S Q L Y

C)

Transition at 13 residues means C to T or conversion will result a stop codon

M T G N Stop

D)

deletion of 7 base will result in frameshift and will code for this amino acid sequence

M T A I N Y

E) Ato T transversion at 15 th base

M T G N H L Y