1 Youre in a research lab and have one sample of 5055uM of 1

1) You\'re in a research lab and have one sample of 50.55uM of 1% MgOH2 that must be tested.Your assay kit gives a range of 3-15nmol. Review the following protocol and decide whether your sample falls within linear range. Will you need more than one sample to fall within this range? If so, how many?

-Dilute 10uL of Magnesium Standard with 990uL of water to prepare a 1.5nmol/uL standard solution.

Add 0,2,3,6, and 10uL of the 1.5nmol/uL standard solution into a 96 well plate, generate blank, 3,6,9,12,15 nmol/well standards. Add water to each well to bring volume to 50uL.

Sample Preparation (Remember insoluble)

-*1-50uL can be added directly to the well

-Bring samples to a final volume with water.

Assay Reaction

-Add 35 uL Magnesium Assay Buffer, 10uL Developer, 5uL of Magnesium Enzyme Mix to each of the wells

-Place wells on horizontal shaker/mix well by pipetting.

-Incubate the reaction for 10min at 37oC. Cover the plate/protect from light during this time.

Measure absorbance at 450nm at initial time (A450)initial. Must be within linear range of curve.

Incubate the plate at 37o throughout measurements. Take measurements every 5min until reach highest (A450) point at 1.5 (A450)final. Continue to protect from light.

Solution

The procedure will give linear range of curve. The assay kit has testing range of 3-15nmol. Concentrations of standards are all right, that will meet the requirements of procedure. Concentrations of substrate will also meet the requirement as it is adding in the amount from 1to 50ul.