Analysis of Functional Groups through Urinalysis Page 3 PreL

Analysis of Functional Groups through Urinalysis Page 3 Pre-Lab Questions Your answers will help you to make predictions about what you will see in the testing stage of lab. The pre-lab assignment will be graded on correctness 1. Leukocytes: What condition does the presence of leukocytes in the urine suggest? Why? 2. Nitrites: What condition does the presence of nitrites in the urine suggest? Why? 3. a Ammonia: What condition does the presence of ammonia in the urine indicate? (Note this will be indicated by measuring the pH of the urine) b. Complete the reaction below to determine how the presence of ammonia impacts the pH of urine. The pH of urine is normally 5.5-6.5 H2O + NH3- c. Will the presence of ammonia increase or decrease the pH of urine? Why?

Solution

1) Leukocytes are the white blood cells that generally fights against any foreign cell invasion or infection in the body.They work with the immune system and fights infection ,and so are dead and disposed off through the urine.Its count in the urine is much higher when the immune system is active during infection.

2)Presence of nitrites in urine indicates bacterial infection of urinary tract as the bacteria converts nitrates into nitrites in the urinary tract that comes out in urine.