1 A genomic library constructed from human brain tissue will

1)

A genomic library constructed from human brain tissue will likely be different from a genomic library constructed from human pancreatic tissue.

1)

Which of the following statements about genomic and cDNA libraries is true?

A)A cDNA library constructed from human brain tissue will likely be different from a cDNA library constructed from human pancreatic tissue. B) Sequences coding for mRNAs are not found in genomic libraries C)At least 95% of a genome is represented in a cDNA library D) A cDNA library would be very useful in examining DNA that does not code for mRNA. E) A genomic library constructed from human brain tissue will likely be different from a genomic library constructed from human pancreatic tissue.

(Problem 4b) The plasmid cloning vector pBR322, shown here, is cleaved with the restriction endonuclease pst1. An isolated DNA fragment from a eukaryotic genome (alco produced by Pgtl cleavage) is added to the prepared vector and ligated. The mixture of ligated DNAs hen used to transf and plasmid-conta ng bacteria ected by gro the pre of tetracyc The cloned DNA fragment is 1,000 bp long and has an EcoRI site 250 bp from one end. Three different recombinant plasmids are cleaved with EcoRI and analyzed by gel electrophonesis, giving the patterns shown below. What does each pattern say about the cloned DNA Note that in peR322, the patl and EcoRI restriction sites are about 750 bp apart. The entire plasmid with no cloned insert has 4,361 bp Size markers in lane 4 have the number of nucleotides noted on the right. 1 2 o The plasmids in lanes 1 and 2 each have one DNA insert, inserted in different orientations. The clone in lane 3 has incorporated two of the DNA fragments, ligated such that the ends farthest from the EcoRI sites are joined. The plasmids in lanes 1 and 2 each have one DNA insert inserted in different orientations. The clone in lane 3 has incorporated two of the DNA fragments, ligated such that the ends closest to the EcoRI sites are joined. The plasmids in lanes 1 and 2 each have one DNA insert, inserted in different orientations. The clone in lane 3 has incorporated two of the DNA fragments, ligated such that both inserts have the same orientation. The plasmids in lanes 1 and 2 each have one DNA insert, inserted in different orientations. The clone in lane 3 has incorporated two or more of the DNA fragments. orientation cannot be determined with multiple inserts.

Solution

Answer: Option A

A. A cDNA library constructed from human brain tissue will likely be different from a cDNA library constructed from human pancreatic tissue.

cDNA library is tissue specific, hence different tissue’s cDNA will differ.

Note: As per the guidelines we can answer only first question. So, I did not answer the remaining questions. Hope you can understand.