can someone help show me how to calculate the volume of wate

can someone help show me how to calculate the volume of water and the volume of albumin stock for the first problem with the BIURET ASSAY

Calculate the volume (1) of the albumin stock (10 mg/mL) and the volume of water (ul.) needed to prepare the standards needed for the Biuret assay (5 standards (prepare 100 L of each standard) containing 2, 4,6, 8 and 10 mg/mL of albumin). Calculate the volume (L) of albumin stock (10 mg/mL) and the volume of water (L) that must be mixed to prepare the standards for the Lowry assay (0.2, 0.4, 0.6, 0.8 and 1 mg/mL) Calculate the volume (L) of albumin stock (10 mg/ml) and the volume of water (L) that must be mixed to prepare 100 L of a 1 mg/mL solution Calculate the volume (L) of 1 mg/mL albumin stock and the volume of water (L) needed to prepare the standards for the Bradford assay (0.02, 0.05, 0.08, 0.11, 0.14 mg/mL)

Solution

1) The concentration of albumin stock in 10 mg/mL and the 5 standard solutions (lets call them S-1, S-2, S-3, S-4 and S-5) required for Biuret assay have albumin concentrations 2, 4, 6, 8 and 10 mg/mL. We have to prepare 100 L of the standard solutions.

Use the dilution equation; take S-1 as an example.

M₁*V₁ = M₂*V₂

where M₁ = 10 mg/mL; M₂ = 2 mg/mL and V₂ = 100 L.

Plug in values and obtain

(10 mg/mL)*V₁ = (2 mg/mL)*(100 L)

====> V₁ = (2 mg/mL)*(100 L)/(10 mg/mL) = 20 L.

Therefore, we shall take 20 L of 10 mg/mL albumn stock and dilute to 100 L in a volumetric flask by adding (100 – 20) L = 80 L water.

Fill in the table as below.

Standard Solution

Concentration of albumin in stock solution (mg/mL)

Volume of albumin stock solution required in L

Volume of water required in L

S-1

2

20

80

S-2

4

40

60

S-3

6

60

40

S-4

8

80

20

S-5

10

100

0

Standard Solution	Concentration of albumin in stock solution (mg/mL)	Volume of albumin stock solution required in L	Volume of water required in L
S-1	2	20	80
S-2	4	40	60
S-3	6	60	40
S-4	8	80	20
S-5	10	100	0