4 You a blank given a bacterial culture which contains 48000

4. You a blank given a bacterial culture which contains 480,000,000 bacteria per mL, and youhave at your an unlimited num volume diagram a serial dilution scheme that will give Using only these dilution colony 1.0 plated onto a colony count within the range when a count you would a Agar plate. Label everything in your diagram, and give the expect to get on the plate. 8 G, o o o o o o e Fulm L.

Solution

Since the broth contains two different types of bacteria. Its imporant to first isolate each of them sepately in different petri dish using selective medium for their growht. Once this done enumeration can be done using counter tool for e.g. Petroff-Hausser counting chamber.

The formula used for the direct microscopic count is:
The number of bacteria per cc = The average numbers of bacteria per large double-lined square X The dilution factors of the large square (1,250,000) X The dilution factor of any dilutions made prior to placing the sample in the counting chamber, e.g., mixing the bacteria with dye

If only viable cells has to be count than viable counting technique can be used.