Which of the following changes in a protein cannot be direct

Which of the following changes in a protein cannot be directed using mass spectrometry (a). Cleavage with an endoprotease (b) Cleavage with an exoprotease (c) Glycosylation (d). Phophorylation (e). all of the above Biopanning involves all except (a) Screening a series of surface protein for ability to bind a target such as an antibody (b). A sequence of 6 histidine at the beginning of each peptide (c) used of a phage display library (d) The bacteriophage 13 (e) All the above are involved in biopanning The significance of metabalomics is (a) It consists of all the small moteailes in the biological system (b) It indicates the flow in the complex net work system with in the cell. (c) It can be studied with in the cell. Followed by chromatography Or NMR (d) All of the above

Solution

1.Option b. Only endoprotease like trypsin are used in mass spec

2. Option B. Polyhistidine tags are used in chromatography to screen and isolate protein. But it is not used in panning.

3. Option A. It is a complete collection of all small molecules in a cell.