Please help Using the table below Of those that recognize of

Please help! Using the table below:

Of those that recognize offset sites and generate staggered cuts, which of these cuts cannot be converted to flush ends by the action of DNA polymerase? & Why? (use answer choices below).

Specificities of some type restriction systems Enzyme Bacterial Source Restriction and Modification Sitea Bacillus amyloliquefaciens H GIGATcc BamHI AN GATCT Bgn B. globiggi GNAATTC EcoR Escherichia coli RY13 EcoRII E. coli R245 CCIGG GGICC Hae Haemophilus aegyptius GAcGCNNNNNI CTGcGNNNNNNNNNN Hgal H. gallinarum GCGIc Hhal H. haemolyticus GTPy. PuAC Hindl H. influenzae Rd H. influenzae Rd AlAGCTT HindIII GNANTc H. influenzae Rf Hinfl GTT AAC Hpa H. parainfluenzae H. parainfluenzae CICGG Hpall clCGG Msp Moraxella sp. GClGGCCGC Notl Nocardia rubra GAGTCNNNN CTCAGN NNNN Ple Pseudomonas lemoignei CTGCANG Pstl Providencia stuartii GN, TCGAC Streptomyces albus G CCCNGGG Sma Serratia marcescens Sb TICTAGA Xbal Xanthomonas badrii

Solution

The restriction enzyme products whose ends cannot be made flush ends are

PstI and HhaI

This is due to the fact that these enzymes make 3\' overhangs after the cleavage, where the DNA polymerase will not have 3 \' hydroxyl group for the extension of the overhang.