HPLC M ethod Development Sample Components H Ho oH Question

HPLC M ethod Development Sample Components - H H-o o-H Question: Please indicate the conditions you would recommend as a starting place for designing the above four molecules. Whe appropriate a separation cf re options are provided please select the one which you feel is most Conditions: Column: C18 Silica C8 Silica Column Size: 4.6 x 15cm2.1x 10cm Detector: ELSD Initial Gradient Condition: Final Gradient Condition: Sample dissolved into: Cyano phase SioH PS-DVB Diode Array MS Injection volume: Your comments on your method development approach:

Solution

Column used must be C18 silica. Size of 4.6*15 cms.

Initial and final gradient = 5-95%

Sample solvent = 50:50 organic:water (methanol or acetonitrile)

Calculate injection volume experimentally.