Experiment testing reaction time blood pressure heart rate a

“Experiment testing reaction time, blood pressure, heart rate, and muscle activity before and after caffeine, where half the subjects have normal cocoa cola and the other half had non caffeinated cocoa cola but non of them know which they got. “

1. (a) Experimental Protocol: how are you going to carry out your experiment(s)?

(a) what are the outcomes / variables that you are going to measure?

(b) how are you going to measure these variables (what is your assay or assays?)

(c) what are your experimental groups? Have you provided adequate control groups?

(d) what reagents will be needed, if any?

(e) what are your approximate sample sizes?

2. Presentation of Results

(a) graphical or tabular analyses: how are you going to present your data?

(b) statistics: how are you going to test whether your variables are significantly different between your experimental groups?

(c) interpretation of results: how will you determine whether your hypothesis or model is supported?

Solution

1. a) Hypothesis: It is hypothesized that normal cocoa cola (with caffeine) consumption would increse the heart rate, blood pressure, muscle activity.

Groups of the experiment: two groups for the experiment is required. Both the groups has 30 participants. 30 subjects have normal cocoa cola(has caffeine) and 30 subjects had non caffeinated cocoa cola. Their heart rate, blood pressure and muscle activity is checked
before and after 15 mins of the consumption.

b) So the outcome variable is
1) Case group (consumed normal cocoa cola)- Blood pressure , Heart rate and muscle activity
2) Control group (consumed non caffeine cocoa cola) -Blood pressure , Heart rate and muscle activity

Blood pressure is check using blood pressure instrument and heart rate is also measured for both the groups, elecctromyography(EMG) is done after the consumption to check the skeletal muscle activity.
Normal blood pressure range : 80/120 mm Hg
Normal heart rate: 70-90 beats / minute

c)Groups

1) Case group (consumed normal cocoa cola)- 30 subjects
2) Control group (consumed non caffeine cocoa cola) -30 subjects

d) Reagent:
1) A sphygmomanometer - Trained persons should check the blood pressure
2) Electromyograph

e)Sample Size:

The \"30\" rule comes from one of the simplest cases: Whether to use a z-test or t-test for comparing two means. A t-test can be done with much less than 30 cases. However, if the expected effect size is small, then the power of such a test will be quite low - that is, it will be unlikely to get a statistically significant result even if the theory is correct.

The approximation becomes better with larger n, given other things remaining the same, thats why I choose 30 subjects per group.