As discussed in lecture bluewhite screening can be used to t

As discussed in lecture, blue-white screening can be used to test for the presence or absence of an insert. The Escherichia coli lacZ gene is 3,072 bp in size. Why is the lacZ gene size a disadvantage when incorporated into a plasmid for blue-white screening?

Solution

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of -galactosidase, an enzyme occurring in E. coli, which cleaves lactose into glucose and galactose. Escherichia coli lacZ gene is 3,072 bp in size. the lacZ gene size a disadvantage when incorporated into a plasmid for blue-white screening have the disadvantagee of not being too flexible in the unique sites in which they have can be used for cloning, and the difficulty of selecting recombinants.