A cross is made between double heterozygous genotypes like a
A cross is made between double heterozygous genotypes, like a Mendelian F1 x F1 (e.g. AaBb x AaBb).
What fraction of the F2 progeny would be expected to show pattern I?
P1NP2 A n B n C n D n E n F G H n ISolution
Lets number each row as 1,2,3,4 in the gel.
Step 1. First we need to find the alleles of a gene. Since parents are heterozygous it is understood that there are 2 genes only.
Lane C shows that 1 and 2 are not alleles. (absence of band)
Lane G shows that 3 and 4 are not alleles. (absence of band)
Lane I shows that 2 and 3 are not alleles (absence of band)
Hence it can be implied that
A. 1 and 3 are alleles
B. 2 and 4 are alleles
Step 2 Lane I observation
Only one band in 1 and 3 - homozygous
Only one band in 2 and 4 - homozygous
Fraction = (1/4) * (1/4) = 1/16
