Whicher primers should we use together Orange 5 GGTCCGCGTTAT

Whicher primers should we use together?

Orange 5\' GGTCCGCGTTATACG 3\' Blue 5\' GCTACAGCATGG 3\' Pink: 5\' CGTAATAA CGCGGACC 3\' Green: 5\' GGATGCTGTAGC 3\' To amplify the following piece of DNA. 5\' GGTCCGcGTTATACGACAT-300bp-CGCTTACTAGCTACAGCATCC 3\' CCAGGCGCATATGCTGTA- TA-300bp- GCGAATGATCGATGTCGTAGG 5\'

Solution

Answer:

The sequence of the Orange primer is 5\' GGTCCGCGTTATACG 3\' and this is complementary to the sequence strand

3\' CCAGGCGCAATATGCTGTA-300bp-GCAATGATCGATGTCGTAGG 5\' - DNA Strand

5\' GGTCCGCGTTATACG 3\' - Primer sequence complementry to DNA strand

The sequence of the pink primer in 3\'-5\' direction is 3 \' CCAGGCGCAATATGC 5\' and is complementry to the strand:

5\' GGTCCGCGTTATACGACAT-300bp-CGCTTACTAGCTAAGCATCC 3\' - DNA Strand

3\' CCAGGCGCAATATGC 5\' - Primer sequence complementry to DNA strand

Therefore Orange and Pink primers should be used so that they can bind to the template by base pairing.