which of the following proteins will elute second from a siz

which of the following proteins will elute second from a size-exclusion column?

Myoglobin

Hemoglobin

GLUT-1

Chymotrypsin

Solution

GLUT-1 will elute second from a size exclusion column.Size exclusion chromatography is a technique used to seperate molecules on the basis of their size.In this technique the bigger molecules move faster while the smaller ones take time and move slower.The molecular weight of myoglobin is 17000 dalton,haemoglobin is 65000 dalton,GLUT -1 is 55000 dalton and chymotrypsin is 25000 dalton.Therefore GLUT-1 will elute second from size exclusion column as it is the second biggest molecule.