Please identify the correct answer Which spectroscopy is mos

Please identify the correct answer:

Which spectroscopy is most useful for determining protein secondary structure Circular Dichroism spectroscopy Mass spectroscopy NMR spectroscopy UV-Visible spectroscopy of Aromatic residues A and C Disulfide bonds in proteins Can load to changes in migration on SDS PAGE Can be exchanged in a redox reaction Can be exchanged in an acid base reaction A and B None of the above X-ray crystallography Allows for convenient assessment of the roles of protons in protein function Has resulted in the structures of some of the largest known supramolecular assemblies Is best for acquiring dynamic information about protein structures Provides similar atomic resolutions in most structures Works best for small highly soluble proteins The 3D structure of proteins Can often tolerate many individual amino acid substitutions. Is often only marginally stable under native conditions Sometimes shows conservation between distantly related proteins that is not obvious in sequence comparison

Solution

5. D

Both circular Dichorism and the NMR used as a tool to predict the secondry structure of a protein.

6.B

Because the disulphide bond links the monomer unit of protein so it can dimerise or trimerise the protein so there will be a shift in SDS-PAGE. Disulfide bonds are usually formed from the oxidation of sulfhydryl (–SH) groups, especially in biological contexts.

7.B

x-ray crystal good details at the smallest scale possible, X-crystallography has no limit (difficulty increases with large complexes), allowing the visualisation of atoms Structure based drug design, High resolution, Mechanisms of action, but it Does not fully reflect the protein dynamics. NMR better for this.

8. B

Protein dynamics can be studied using NMR, while X-ray crystallography gives static structural information.

8.